[abstract] Neutrophil activation status in Navy clearance divers following decompression in trimix dives with in-water oxygen decompression

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[abstract] Neutrophil activation status in Navy clearance divers following decompression in trimix dives with in-water oxygen decompression

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dc.contributor.author Cameron, BA en_US
dc.contributor.author Rhind, SG en_US
dc.contributor.author Holden, N en_US
dc.contributor.author Eaton, DJ en_US
dc.contributor.author Nishi, RY en_US
dc.contributor.author Bouak, F en_US
dc.contributor.author Bateman, WA en_US
dc.contributor.author McLellan, TM en_US
dc.date.accessioned 2012-06-03T20:30:04Z
dc.date.available 2012-06-03T20:30:04Z
dc.date.issued 2011 en_US
dc.identifier.citation Undersea Hyperb Med. 2011 Sep-Oct;38(5) en_US
dc.identifier.isbn 1066-2936 en_US
dc.identifier.uri http://archive.rubicon-foundation.org/9943
dc.description Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc. en_US
dc.description.abstract Background: Immunoinflammatory response (IR) may play an important role in the evolution of decompression stress to decompression sickness (DCS). It is thought that an important IR pathway exists during decompression stress when venous endothelial cells (ECs), mechanically disturbed by venous gas emboli (VGE), express a broad spectrum of intercellular messengers that systemically activate platelets, peripheral blood cells and other ECs. DCS onset may involve a cascade of messengers produced by activated cells within the vasculature similar to systemic inflammatory response syndrome in sepsis. Neutrophil activation is a hallmark of innate immune function and may serve as an important diagnostic marker of DCS. Methods: Using multicolor flow cytometry and antibodies against nine specific neutrophil surface proteins, we examined the activation status of neutrophils in navy clearance divers (n=8) following decompression (DCIEM decompression algorithm) in a range of trimix dives that all included in-water O2 decompression (IWO2). Results: Many activation markers were affected by the dives. For example, the surface density of two neutrophil- EC adhesion molecules, CD62L (neutrophil capture) and CD11b (neutrophil 2-Integrin), were significantly reduced following the dive to 69 msw for 20 minutes. Moreover, the neutrophil surface protein marker, CD162, known to interact with CD62L during IR, showed a similar trend after the 69-msw dive. Indeed, the surface density of six of the nine CDs examined in the neutrophils of clearance divers was significantly reduced after this dive. Summary: Although the 69 msw dive generated very high levels of VGE, the low surface densities of CD62L, CD11b, CD162 and three other CDs suggest that a component of the dive suppressed neutrophil activation. A possible mechanism for suppressed neutrophil activation during IWO2 could be similar to that reported during medical HBO2T where IR is involved. Neutrophil activation status may be useful for diagnosis of DCS and prediction of ideal decompression schedules. en_US
dc.language.iso en en_US
dc.publisher Undersea and Hyperbaric Medical Society, Inc. en_US
dc.subject decompression sickness en_US
dc.subject DCS en_US
dc.subject Neutrophil activation en_US
dc.subject Immunoinflammatory response en_US
dc.subject endothelial cells en_US
dc.subject bubbles en_US
dc.subject in-water oxygen decompression en_US
dc.subject human en_US
dc.title [abstract] Neutrophil activation status in Navy clearance divers following decompression in trimix dives with in-water oxygen decompression en_US
dc.type Article en_US

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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