[abstract] THE EFFECTS OF INCREASED PRESSURES OF HELIUM AND THE NARCOTIC GASES NITROGEN AND ARGON ON CALCIUM UPTAKE BY ACTIVATED NEUROBLASTOMA CELLS.

Rubicon Research Repository/Manakin Repository

[abstract] THE EFFECTS OF INCREASED PRESSURES OF HELIUM AND THE NARCOTIC GASES NITROGEN AND ARGON ON CALCIUM UPTAKE BY ACTIVATED NEUROBLASTOMA CELLS.

Show simple item record


dc.contributor.author Philp, RB
dc.contributor.author Kalogeros, G
dc.date.accessioned 2009-09-24T02:57:00Z
dc.date.available 2009-09-24T02:57:00Z
dc.date.issued 1993
dc.identifier.uri http://archive.rubicon-foundation.org/8201
dc.description Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held July 7-10, 1993. World Trade and Convention Centre, Halifax, Nova Scotia, Canada. (http://www.uhms.org) en
dc.description.abstract Cultured human neuroblastoma SK-N-SH cells were loaded with the intracellular fluorescent Ca2+ indicator fura-2 AM and either stimulated with carbachol (the cells express M3 type muscarinic receptors) or depolarized with 50 mM KCl. Both evoked a rapid, marked increase in free cytosolic Ca2+ [Ca2+]i determined by fluorescence spectroscopy. Studies in Ca2+-free medium indicated that about 65% of this increase was from uptake of extracellular Ca2+. Non-narcotic He, 9-36 ATA, significantly increased evoked peak [Ca2+]i in response to carbachol or KC1 in a pressure-dependent fashion; eg with carbachol, mean +- sem was 378.8 +- 19.6 for 1 ATA control vs. 481.7 nM +- 24.4 for 36 ATA He (p<.003 by ANOVA). At the same pressures neither N2, nor Ar which is about 2x as narcotic as N2, changed [Ca2+]i significantly from control values, thus apparently negating the pressure effect represented by He. With Ar a slight but not statistically-significant decline from control was seen eg 368.2 +- 31.1 for control, 344.8 +- 16.7 for 36 ATA Ar. Since a rise in intracellular Ca2+ is associated with neuronal activation, these results suggest that changes in Ca2+ fluxes may underly HPNS in divers and its antagonism by narcotic gases. Supported by a grant from the U.S. Office of Naval Research. en
dc.format.extent 258 bytes
dc.format.mimetype text/plain
dc.language.iso en en
dc.publisher Undersea and Hyperbaric Medical Society, Inc. en
dc.subject HBO en
dc.subject hyperbaric oxygenation en
dc.subject Helium en
dc.subject Nitrogen en
dc.subject argon en
dc.subject calcium en
dc.subject neuroblastoma en
dc.subject human en
dc.subject narcosis en
dc.title [abstract] THE EFFECTS OF INCREASED PRESSURES OF HELIUM AND THE NARCOTIC GASES NITROGEN AND ARGON ON CALCIUM UPTAKE BY ACTIVATED NEUROBLASTOMA CELLS. en
dc.type Other en

Files in this item

Files Size Format View
abstract.txt 258bytes Text file View/Open

This item appears in the following Collection(s)

  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

Show simple item record

Browse

My Account