[abstract] HBO AND THE EFFECT OF BROWN RECLUSE SPIDER VENOM IN RABBITS.

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[abstract] HBO AND THE EFFECT OF BROWN RECLUSE SPIDER VENOM IN RABBITS.

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Title: [abstract] HBO AND THE EFFECT OF BROWN RECLUSE SPIDER VENOM IN RABBITS.
Author: Maynor, ML; Moon, RE; Klitzman, B; Fracica, PJ; Canada, A
Abstract: BACKGROUND: L. Reclusa (brown recluse) spider bites can produce severe skin lesions which may require extensive surgical repair. Several reports suggest that hyperbaric oxygen (HBO) may be of benefit in healing these lesions. METHODS: 37 New Zealand white rabbits received 58 intradermal injections of 73 microliters of raw venom extract mixed with physiologic buffered saline (Dulbeccos solution). Control injections were made with buffer. Animals were divided into 6 groups: (1) venom and no HBO, (2) venom and one immediate HBO treatment (100% O2), (3) venom and immediate HBO for 10 treatments (100% O2), (4) venom and then delayed HBO (48 hrs) for 10 treatments (100% O2), (5) venom and immediate HBO for 10 treatments (9% O2), (6) sodium citrate buffer injections and immediate HBO for 10 treatments (100% O2). HBO treatments were at 2.5 atmospheres for 90 minutes twice daily. Daily measurements were made of the lesion diameter, and skin blood flows utilizing a laser doppler probe. RESULTS: are summarized in the following table. WOUND DIAMETER (cm) [table 1]: Group, Day 0, Day 3, Day 7; Controls: 0, 3.4 +- .6, 4.0 +- .6; Immed HBO x 10: 0, 1.9 +- 4, 1.3 +- .6; Immed HBO (9% O2): 0, 2.2 +- .9, 3.8 +- 1.0; Delay HBO x 10: 0, 2.1 +- .6, 1.4 +- .7; Immed HBO x 1: 0, 1.7 +- .6, 2.1 +- .9; Citrate: 0, 0.0 +- 0.0, 0.0 +- 0.0; [end table 1] BLOOD FLOW (arbits) [table 2] (Mean +- 95% c.i.); Group, Day 0, Day 3, Day 7; Controls: 16.9 +- 6.1, 1.4 +- 1.1, .1 +- .1; Immed HBO x 10: 28.7 +- 16.6, 1.0 +- 1.5, 4.4 +- 5.4; Immed HBO (9% O2): 18.5 +- 6.6, 1.6 +- 1.9, .2 +- .4; Delay HBO x 10: 15.3 +- 6.4, 5.8 +- 4.3, 7.1 +- 19.4; Immed HBO x 1: 22.7 +- 11.8, 2.6 +- 3.4, 1.4 +- 1.1; Citrate: 37.5 +- 30.6, 21.0 +- 19.3, 12.7 +- 25.3 [end table 2]; There was a statistically significant effect of HBO treatment on wound diameter (P = .0001, ANOVA). HBO significantly decreased wound diameter irrespective of the protocol used. There was no significant effect of HBO on wound blood flow. Histologically, there was more evidence of lesion healing in all HBO treated groups than the controls. CONCLUSION: HBO significantly rcduces the size of lesions caused by L. Reclusa. In this model a single HBO exposure is as effective as multiple treatments.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held July 7-10, 1993. World Trade and Convention Centre, Halifax, Nova Scotia, Canada. (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/8138
Date: 1993

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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