[abstract] ENHANCED NUCLEAR FACTOR (NF)-kappaB ACTIVATION IN EXPERIENCED DIVERS IN RESPONSE TO ACUTE HYPERBARIC STRESS.

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[abstract] ENHANCED NUCLEAR FACTOR (NF)-kappaB ACTIVATION IN EXPERIENCED DIVERS IN RESPONSE TO ACUTE HYPERBARIC STRESS.

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Title: [abstract] ENHANCED NUCLEAR FACTOR (NF)-kappaB ACTIVATION IN EXPERIENCED DIVERS IN RESPONSE TO ACUTE HYPERBARIC STRESS.
Author: Rhind, SG; Cameron, BA; Eaton, DJ; McLellan, TM
Abstract: BACKGROUND: Nuclear factor (NF)-kB is a master transcriptional switch in inflammation, immunity and apoptosis. Activated NF-kB migrates into the nucleus to regulate the expression of multiple target genes influencing many aspects of normal and disease physiology. The aim of this study was to investigate nuclear translocation of NF-kB within circulating leukocytes in subjects exposed to progressive levels of hyperbaria. MATERIALS & METHODS: Naive subjects (N, n=9) and experienced divers (D, n=12) underwent 30-min hyperbaric exposures at 180 kPa (18 msw; low stress); 300 kPa (30 msw; moderate stress); 450 kPa (45 msw; high stress)] separated by 1-week intervals. Peripheral blood samples were drawn pre-dive, 20 and 60-min post-dive. A whole blood flow cytometric assay was used to quantify spontaneous and in vitro LPS-stimulated NF-kB translocation in peripheral blood mononuclear cells (PBMC) and neutrophils (PMNs). Results are expressed as the mean % positive and median fluorescence intensity (MFI) of each population. RESULTS: Resting spontaneous expression of NF-kB in PBMC and PMNs was similar between N and D. Following acute hyperbaric stress, D exhibited a significant (p < .05) time-dependent elevation (up to 60%) of intranuclear NF-kB expression (% and MFI) in both PBMC and PMNs at all depths; whereas, significant translocation occurred only after 45 m, 60-min post-dive in N. Subsequent LPS stimulation induced significantly greater increases in both pre-dive (20%) and 20-min post-dive (45%) levels of NF-kB translocation in D compared to N responses. CONCLUSIONS: This study demonstrates enhanced nuclear translocation of NF-kB in humans following acute hyperbaric exposure that is upregulated in experienced divers following repetitive episodes of hyperbaric stress. These results suggest that modulation of NF-kB-dependent pathways may contribute to diving acclimatization and a reduced susceptibility to decompression sickness in individuals undergoing chronic hyperbaric stress.
Description: Abstract of the Undersea & Hyperbaric Medical Society 2008 Annual Scientific Meeting June 26-28, 2008 Salt Lake City Marriott Downtown, Salt Lake City, Utah.
URI: http://archive.rubicon-foundation.org/7796
Date: 2008

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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