[abstract] INFLAMMATORY GENE EXPRESSION IN THE LEUKOCYTES OF NAIVE SUBJECTS AND EXPERIENCED DIVERS FOLLOWING ACUTE HYPERBARIC STRESS.

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[abstract] INFLAMMATORY GENE EXPRESSION IN THE LEUKOCYTES OF NAIVE SUBJECTS AND EXPERIENCED DIVERS FOLLOWING ACUTE HYPERBARIC STRESS.

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Title: [abstract] INFLAMMATORY GENE EXPRESSION IN THE LEUKOCYTES OF NAIVE SUBJECTS AND EXPERIENCED DIVERS FOLLOWING ACUTE HYPERBARIC STRESS.
Author: Cameron, BA; McLellan, TM; Eaton, DJ; Rhind, SG
Abstract: BACKGROUND: Peripheral blood leukocytes can be exploited as windows of the inflammatory response as they use cellular multi-pathway mechanisms to scrutinize, respond and orchestrate adaptive tolerance during physiological stress such as decompression sickness (DCS). mRNA microarray (MA) and flow cytometric (FC) analyses were used to monitor genomic transcription and protein expression in leukocytes of experienced divers (ExpD) and naive subjects (NS) following hyperbaric stress. MATERIALS AND METHODS: Peripheral blood samples were drawn from NS (n=6) and ExpD (n=6) pre and post-60 min after surfacing from hyperbaric stress exposure at 45 msw for 30 min. Expression levels of purified leukocyte mRNA were screened for a range of inflammatory markers using Agilent two-colour MA analysis and normalized to mRNA levels from quiescent cells. Monoclonal antibodies against several inflammatory markers used with whole blood FC assays quantified mean % positive and median fluorescence intensity (MFI) in peripheral blood monocytes and neutrophils. RESULTS: FC analysis showed increased apoptosis in the leukocytes of NS at post-60 but no change in ExpD. In contrast, HSP70 in the neutrophils and monocytes in ExpD increased at post-60 but was unchanged in NS. MA analysis revealed significantly higher levels of HSP70B’ mRNA in the leukocytes of ExpD compared with NS. Post-dive mRNA expression levels of several prominent leukocyte pro and anti-apoptotic inflammatory markers were also significantly altered in both groups. CONCLUSIONS: Acute hyperbaric stress differentially modulated genome-wide mRNA expression and cellular functionality in ExpD versus NS. Future studies will integrate mRNA MA and cell-specific pathway FC analyses with the goal of identifying specific markers of susceptibility and/or diagnosis of DCS.
Description: Abstract of the Undersea & Hyperbaric Medical Society 2008 Annual Scientific Meeting June 26-28, 2008 Salt Lake City Marriott Downtown, Salt Lake City, Utah.
URI: http://archive.rubicon-foundation.org/7795
Date: 2008

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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