[abstract] HBO DECREASES ENDOTHELIAL CELL INTERCELLULAR ADHESION MOLECULE - 1 (ICAM-1) PROTEIN EXPRESSION AND NEUTROPHIL ADHESION IN AN IN-VITRO MODEL OF ISCHEMIA / REPERFUSION.

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[abstract] HBO DECREASES ENDOTHELIAL CELL INTERCELLULAR ADHESION MOLECULE - 1 (ICAM-1) PROTEIN EXPRESSION AND NEUTROPHIL ADHESION IN AN IN-VITRO MODEL OF ISCHEMIA / REPERFUSION.

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Title: [abstract] HBO DECREASES ENDOTHELIAL CELL INTERCELLULAR ADHESION MOLECULE - 1 (ICAM-1) PROTEIN EXPRESSION AND NEUTROPHIL ADHESION IN AN IN-VITRO MODEL OF ISCHEMIA / REPERFUSION.
Author: Buras, JA; Reenstra, WR; Svoboda, KS
Abstract: BACKGROUND: ICAM-1 is required for neutrophil (PMN) transmigration across the endothelium during ischemia reperfusion (IR) injury. We hypothesize that HBO might down-regulate ICAM-1 production and this explain the in-vivo findings that HBO decreases neutrophil adhesion in I/R injury. METHODS: Endothelial cells from bovine aortas or human umbilical veins were exposed to 4 hours of hypoxia and hypoglycemia to simulate ischemia followed by normoxia/normoglycemia for a total of 24 hours. Alternatively, cells were treated with HBO at 2.5 ATA for 90 min following mock ischemia, prior to mock reperfusion. At 24 hours ICAM-1 protein expression was analyzed using confocal microscopy (CM), cell surface ELISA, or western blot analysis. PMN adhesion to live endothelial cells was also performed. RESULTS: We have found that ICAM-1 upregulation required exposure to hypoxia and hypoglycemia. HBO treatment of cells exposed to hypoxia/hypoglycemia down-regulated cell surface and total ICAM-1 protein expression to control levels as determined by CM, ELISA, and western blotting. HBO treatment of cells subjected to I/R decreased PMN adhesion to control levels. CONCLUSION: ICAM-1 is up-regulated in an in-vitro model of endothelial cell I/R injury. The increase in ICAM-1 and PMN adhesion is inhibited by HBO treatment of endothelial cells. this suggests that the beneficial mechanism of HBO in treating I/R injury may be mediated through regulation of endothelial cell adhesion molecules.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/731
Date: 1998

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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