[abstract] HARMFUL EFFECTS OF HYPERBARIC OXYGEN ON THE RAT KIDNEY FOLLOWING GLOBAL ISCHEMIA.

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[abstract] HARMFUL EFFECTS OF HYPERBARIC OXYGEN ON THE RAT KIDNEY FOLLOWING GLOBAL ISCHEMIA.

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Title: [abstract] HARMFUL EFFECTS OF HYPERBARIC OXYGEN ON THE RAT KIDNEY FOLLOWING GLOBAL ISCHEMIA.
Author: Riseman, JA; Schultz, R; Roth, AC; Mody, N; Mathur, A; Birtch, AG
Abstract: Oxygen-derived free radicals have been shown to cause injury secondary to ischemia/ reperfuslon in multiple organ systems. Free radicals scavengers, including superoxide dismutase (SOD) have been shown to reduce the effects of renal ischemia. We have shown that hyperbaric oxygen treatment (HBO) reduces the injury of axial skin flaps following ischemia. We theorized that HBO combined with SOD treatment may reduce Ischemic/ reperfusion injury of rat kidneys. Male Sprague-Dawley rats weighing 225-275 grams underwent celiotomy. The rats were anesthetized and the left renal artery clamped with a nontraumatlc 3 mm microsurgery clamp for 60 minutes. Contralateral nephrectomy was performed. Four groups were studied. The control group (n=10) was exposed to 60 minutes of ischemia only. Another group (n=10) was treated with HBO before ischemia (HBOB). The third group (n=11) received HBO after ischemia (HBOA). The final group (n=12) was treated with HBO after ischemia and SOD (20,000 u/kg) 2 minutes before reperfusion (HBOA+SOD). HBO treatments were for 105 minutes at 2.5 ata and 100% oxygen. Baseline serum creatinine levels were obtained at celiotomy and follow up levels measured on days 1, 2, 5, 9, and 14 days. Rats were euthanlzed on day 14 and the kidneys removed for histopathologic exam. Creatinine levels were normalized to baseline levels for each rat with positive changes in creatinine output indicating impaired kidney function. The greatest changes in creatinine output in the control group were seen in the first two days following injury, indicating an acute injury or "renal stunning". The HBOA groups showed significantly greater creatinine output as compared to the controls at day 1, 2.8 VS. 1.1 (p<.05), and on day 2, 3.2 vs. 0.7 (p<.05). on day 5 this HBOA treated group was not different from control and by day 9 all groups had returned to baseline levels. The HBOB group showed lower creatinine levels only on day 5 following ischemia, 0.3 vs 0.9 (p<.05). The HBOA+SOD animals had creatinine levels significantly less than the HBOA group but not less than the control group. On histological examination all kidneys were similar with proximal tubules seen in various stages of regeneration. HBO pretreatment does not significantly improve the recovery of kidneys from 60 minutes of ischemia. HBO given during the reperfusion of kidneys may exacerbate the production oxygen-derived free radicals and reperfusion injury.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Joint Annual Scientific Meeting with the International Congress for Hyperbaric Medicine and the European Undersea Biomedical Society held 11-18 August 1990. Okura Hotel, Amsterdam, The Netherlands (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/7020
Date: 1990

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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