[abstract] HYPERBARIC OXYGEN THERAPY FOR ISCHEMIC STROKE: MECHANISMS AND MULTIPLE APPLICATIONS

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[abstract] HYPERBARIC OXYGEN THERAPY FOR ISCHEMIC STROKE: MECHANISMS AND MULTIPLE APPLICATIONS

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Title: [abstract] HYPERBARIC OXYGEN THERAPY FOR ISCHEMIC STROKE: MECHANISMS AND MULTIPLE APPLICATIONS
Author: Calvert, J; Yin, D; Zhou, C
Abstract: BACKGROUND: The mechanisms of HBO in acute cerebral ischemic stroke and the advantage of multiple HBO applications were studied. METHODS: Adult male Sprague-Dawley rats were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R) and subsequently exposed to HBO [2.5 atmospheres absolute (ATA)] for 2 hours either at 6 hours or 24 hours after MCAO/R and was repeated daily for 6 days. Rats with 6 or 24 hour-delayed HBO after MCAO/R displayed a significant decrease of infarct ratio and amelioration of neurological deficits compared to the MCAO/R-untreated group. RESULTS: This study suggests that delayed, but multiple HBO treatments can improve neurological evaluation and reduce cerebral infarction. In another series studies, rats were treated with a single HBO for 2 hrs and were sacrificed and brain samples were collected at 24, 48, 72 hrs and 7 days after reperfusion for studying apoptosis. In MCAO/R without HBO treatment animals, DNA fragmentation was observed in injured cortex at 24, 48 and 72 hr but not in samples at 7 days after reperfusion. Double labeling of brain slides with NeuN and caspase-3 demonstrated neurons in the injured cortex labeled with caspase-3. TUNEL+H and E co-staining revealed morphological apoptotic changes at 24, 48 and 72 hrs after reperfusion. HBO abolished DNA fragmentation and reduced the number of TUNEL positive cells. HBO reduced infarct area and improved neurological scores at 7 days after reperfusion. CONCLUSIONS: One of the molecular mechanisms of HBO-induced brain protection is to prevent apoptosis and this effect of HBO might preserve more brain tissues and promote neurological functional recovery.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/1565
Date: 2004

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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