[abstract] IN HUMAN DERMAL FIBROBLASTS INCREASED EXPRESSION OF GROWTH FACTOR RECEPTORS IN RESPONSE TO HYPERBARIC OXYGEN IS NITRIC OXIDE DEPENDENT

Rubicon Research Repository/Manakin Repository

[abstract] IN HUMAN DERMAL FIBROBLASTS INCREASED EXPRESSION OF GROWTH FACTOR RECEPTORS IN RESPONSE TO HYPERBARIC OXYGEN IS NITRIC OXIDE DEPENDENT

Show full item record


Title: [abstract] IN HUMAN DERMAL FIBROBLASTS INCREASED EXPRESSION OF GROWTH FACTOR RECEPTORS IN RESPONSE TO HYPERBARIC OXYGEN IS NITRIC OXIDE DEPENDENT
Author: Reenstra, WR; Orlow, DL; Buras, JA
Abstract: Objectives: Human dermal fibroblasts have several functions in the maintenance of skin integrity. Fibroblasts proliferate in response to growth factors, differentiate and secrete matrix molecules in response to extracellular signals. There is a profound decrease in wound healing rates in patients with vascular insufficency. We have demonstrated previously that a single HBO treatment increases both cellular proliferation and growth factor (GF) receptor levels. This study hypothesizes that a HBO increases GF receptor levels via a nitric oxide dependent mechanism. Methods: Skin dermal fibroblasts from normal healthy volunteers and those with Type II Diabetes were derived from primary explants. At low passage levels cells were divided into the following groups: (A) 48 hours of standard tissue culture conditions (STC) [5percentCO2 + Room air] (B) 48 hours of STC conditions with nitric oxide inhibitors, (C) 46.5 hours of STC with 90 min of 100percent O2 at 2.5ATA or (D) 46.5 hours of STC with 90 min of 100percent O2 at 2.5ATA and nitric oxide inhibitors. Cellular proliferation rates were determined on all four groups by growth curves. Western analysis, ELISA and confocal microscopy assessed growth factor receptor expression. Cellular signaling was determined by western and confocal analysis Results: Fibroblasts in the HBO treated group had a significantly faster proliferation rate than STC (30+4 hrs; 17+1hrs, p less than 0.01). The increased proliferation was reversed with the inhibition of nitric oxide. Growth factor receptor levels were significantly increased in hyperbaric treated groups as compared to the STC. (1.25x 105 versus 7.8x104 p less than 0.01). The measured increase in receptor number after HBO was reversed when nitric oxide was inhibited. Conclusions: These studies suggest that skin fibroblasts treated with HBO have a increased rate of proliferation and responsiveness to growth factors because of a increase in growth factor receptor expression and signaling. HBO appears to be modifying growth factor receptor number through a nitric oxide dependent mechanism Ultimately this information will provide the basis for understanding the role of oxygen in fibroblast response and migration in wound healing.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/1193
Date: 2002

Files in this item

Files Size Format View
abstract.txt 259bytes Text file View/Open

This item appears in the following Collection(s)

  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

Show full item record

Browse

My Account