[abstract]HYPERBARIC OXYGEN PRECONDITIONING INCREASES TOLERANCE TO DECOMPRESSION SICKNESS VIA HSP70 IN RATS

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[abstract]HYPERBARIC OXYGEN PRECONDITIONING INCREASES TOLERANCE TO DECOMPRESSION SICKNESS VIA HSP70 IN RATS

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Title: [abstract]HYPERBARIC OXYGEN PRECONDITIONING INCREASES TOLERANCE TO DECOMPRESSION SICKNESS VIA HSP70 IN RATS
Author: Xu WG; Ni XX; Fan DF; Sun Q; Kang ZM; Cai ZY
Abstract: Introduction/Background: Decompression sickness (DCS) is a major concern in diving. Hyperbaric oxygen (HBO2) preconditioning has been proved to enhance tolerance to DCS via nitric oxide. Heat-shock protein (HSP) 70 was also found to have protective effects on DCS. We hypothesized that the beneficial effects of HBO2 preconditioning on DCS was related with HSPs. Materials and methods: HSPs (70, 27 and 90) expressed in tissues of spinal cord and lung in rats were detected at different time points following HBO2 exposure by western blot. Rats were subject to a simulated air dive and rapid decompression to induce DCS at the 18-hour time point following HBO2 exposure. Bubbles in pulmonary artery were detected using ultrasound imaging to observe the possible effect of HBO2 preconditioning on DCS bubble formation. The severity of DCS, HSP70 expression, oxidative and apoptotic parameters in the lung and spinal cord were compared among different groups of rats pretreated with HBO2, HBO2 plus L-NAME, HBO2 plus quercetin, or normobaric air. Results: HSP70 increased and reached highest at 18 hours following exposure. The amounts of bubbles in rats pretreated with HBO2 or air showed no difference. HBO2 preconditioning significantly reduced the morbidity of DCS (from 66.7 percent to 36.7 percent), the increased levels of oxidation and apoptosis. L-NAME or quercetin eliminated most of the beneficial effects of HBO2 on DCS, and counteracted the stimulation of HSP70 by HBO2 Summary/Conclusions: These results indicated that HSP70 was involved in the beneficial effects of HBO2 on DCS, mainly by the anti-oxidation and anti-apoptosis effects.
Description: Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/10439
Date: 2012

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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