[abstract] EFFICACY OF HYPERBARIC OXYGEN THERAPY IN A MOUSE MODEL OF IMPLANT-ASSOCIATED OSTEOMYELITIS

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[abstract] EFFICACY OF HYPERBARIC OXYGEN THERAPY IN A MOUSE MODEL OF IMPLANT-ASSOCIATED OSTEOMYELITIS

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Title: [abstract] EFFICACY OF HYPERBARIC OXYGEN THERAPY IN A MOUSE MODEL OF IMPLANT-ASSOCIATED OSTEOMYELITIS
Author: Shandley, S; Prato-Matthews, K; Cox, J; Abplanalp, A; Romano, D; Kalns, J; Michaelson, R
Abstract: Introduction: A major concern for wounded soldiers is the threat of infection of the bone, or osteomyelitis. Ballistic projectiles cause trauma which may introduce bacteria into bones and surrounding tissue. Additionally, surgical procedures and orthopedic implants required to mend injured bones may increase exposure to bacterial pathogens. Osteomyelitis is difficult to treat with antibiotics alone and results in significant morbidity, complications, and increased hospitalization time. We hypothesized that a mouse model for implant-associated osteomyelitis can be used to evaluate the efficacy of hyperbaric oxygen therapy (HBOT) in the prevention and/or resolution of infections with deployment-related bacteria, including methicilin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae. Materials and Methods: MRSA, P. aeruginosa, and K. pneumoniae were isolated from wounded soldiers. Clinical isolates were grown in liquid cultures overnight and then used to coat stainless steel pins that were inserted into the tibias of C57BL/6 mice to produce chronic osteomyelitis infections. Mice received HBOT (100% oxygen at 2.4 ATA for 80 minutes) prophylactically for 5 days prior to infection followed by 12 or 19 days of HBOT; or mice received HBOT starting 5 days post-infection for 7 or 14 days. Bone lesion severity and bacterial burdens were compared between groups that received HBOT and controls groups that did not receive HBOT. Results: The rates of infection for MRSA, P. aeruginosa, and K. pneumoniae were 80%, 63%, and 34% and the median bacterial burdens were 9.2x106, 5.7x105, and 6.0x105 colony forming units/gram of tibia, respectively. Lesion morphology differed between the bacteria: MRSA induced abscesses and severe lesions, P. aeruginosa induced moderate to severe lesions, and K. pneumoniae induced mild to moderate lesions. Summary: This model can be used to evaluate the efficacy of HBOT in a mouse model of implant-associated osteomyelitis with clinical isolates of MRSA, P. aeruginosa, and K. pneumoniae.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting, St Pete Beach, Florida, USA. (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/9277
Date: 2010

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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