[abstract] THE ABSENCE OF INNATE INFLAMMATORY GENE RESPONSE TO ACUTE HYPERBARIC STRESS IN NON-DIVERS FOLLOWING HEAT ACCLIMATION

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[abstract] THE ABSENCE OF INNATE INFLAMMATORY GENE RESPONSE TO ACUTE HYPERBARIC STRESS IN NON-DIVERS FOLLOWING HEAT ACCLIMATION

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Title: [abstract] THE ABSENCE OF INNATE INFLAMMATORY GENE RESPONSE TO ACUTE HYPERBARIC STRESS IN NON-DIVERS FOLLOWING HEAT ACCLIMATION
Author: Cameron, B; McLellan, T; Eaton, D; Rhind, S
Abstract: BACKGROUND: Using microarray analysis in a previous study we demonstrated significantly increased mRNA transcription of several inflammatory genes in the peripheral blood leukocytes of experienced divers over that in non-divers. Recent reports that heat acclimation and/or exercise training reduces hyperbaric stress, in conjunction with the growing body of evidence showing a common inflammatory response to various forms of stress prompted the following hypothesis. Heat acclimation would modify the inflammatory gene response to hyperbaric stress in non-divers. MATERIALS AND METHODS: mRNA was isolated from peripheral blood leukocytes in non-divers (n=9) pre and post-60 min after surfacing from hyperbaric exposure at 18 msw for 80 min before and after a 9-day heat acclimation protocol known to promote rheological and physiological adaptations in humans. Transcribed mRNA levels in non-divers were screened for a range of inflammatory markers using Agilent two-colour microarray analysis and normalized to mRNA levels from quiescent reference cells. Venous gas emboli were measured (K-M scoring) using Doppler ultrasound. RESULTS: Doppler Ultrasound-detected bubble scores were low in all but one subject. Microarray analysis showed no significant differences in mRNA transcription levels of inflammatory genes including HSP70B' and TNF-¥ after acute hyperbaric exposures before and after heat acclimation. CONCLUSIONS: These findings are consistent with our previous results showing that, compared to experienced divers non-divers exhibit little or no change in mRNA transcription levels upon exposure to hyperbaric-decompression stress. Moreover, 9 days of heat acclimation did not significantly modify the pattern of gene expression observed in their blood leukocytes. The results did not support the hypothesis that heat acclimation would induce cross-adaptation of the inflammatory gene response to hyperbaric stress in non-divers.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting, Las Vegas, Nevada, USA. (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/9179
Date: 2009

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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