[abstract] THE IMPACT OF HYPERBARIC OXYGEN THERAPY ON SURVIVAL IN MUCORMYCOSIS: PRELIMINARY RESULTS OF AN ON-GOING RANDOMIZED CONTROLLED TRIAL IN THE MURINE MODEL.

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[abstract] THE IMPACT OF HYPERBARIC OXYGEN THERAPY ON SURVIVAL IN MUCORMYCOSIS: PRELIMINARY RESULTS OF AN ON-GOING RANDOMIZED CONTROLLED TRIAL IN THE MURINE MODEL.

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Title: [abstract] THE IMPACT OF HYPERBARIC OXYGEN THERAPY ON SURVIVAL IN MUCORMYCOSIS: PRELIMINARY RESULTS OF AN ON-GOING RANDOMIZED CONTROLLED TRIAL IN THE MURINE MODEL.
Author: Barratt, DM; Asmar, P; Nolan, TA; Trahan, C; Metzinger, S; Van Meter, KW
Abstract: BACKGROUND: Mucormycosis is a rare fungal infection occurring in severely immunocompromised individuals. Amphotericin B and surgical debridement are the mainstays of treatment. Overall mortality ranges from 40-80percent, depending on the underlying disease. METHODS: IACUC approval was obtained prior to beginning the study. On day 1, sixty female CD-1 mice were pretreated with 5mg of deferoxamine. Three hours later, they were anethetized and innoculated with 10'6 spores of Rhizopus arrhizus via both the tail vein and the right ethmoid sinus. Group 1 (n=5) received no treatment. Remaining animals were randomized and treatment was bugun immediately. Group 2 (n=27) received daily intraperitoneal Amphotericin B (0.31 mg/kg) and twice daily hyperbaric oxygen treatments (100percent O2 at 33 fsw for 2 hours). Group 3 (n=28) received daily intraperitoneal Amphotericin B (0.31 mg/kg) and twice daily sham treatments (21percent O2 at 5 fsw for 2 hours). On day 14, treatments were discontinued and all surviving animals were sacrificed. The following organs of animals from Group 1 were sent to pathology for HandE and PAS staining and analysis: heart; lungs; brain;kidneys; spleen; and liver. RESULTS: Within 36 hours, some mice demonstrated an overall decrease in mobility, cessation of normal activities, visible lesions about the orbit, face, or nose, and hemiplegia or paraplegia. All animals in Group 1 died within one week. Histopathologic analysis revealed fungal elements in the brains and kidneys. A generalized pneumonia was also detected, but fingal elements were not detected in the lungs. Survival on day 14 was 96percent (26/27) in Group 2 and 82percent (23/28) in Group 3. A one-tailed Fisher's exact use test (p=0.10) did not reach statistical significance. CONCLUSION: Cause of death in this murine model of mucormycosis was thought to be a combination of fungal encephalitis and neurologic respiratory depression. Although the addition of hyperbaric oxygen therapy to Amphotericin B improved survival in this murine model of mucormycosis, the study did not reach statistical significance.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/795
Date: 1999

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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