[abstract] MACROPHAGE-LIKE PROPERTIES OF RETROVIRALLY-TRANSFORMED CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS.

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[abstract] MACROPHAGE-LIKE PROPERTIES OF RETROVIRALLY-TRANSFORMED CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS.

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Title: [abstract] MACROPHAGE-LIKE PROPERTIES OF RETROVIRALLY-TRANSFORMED CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS.
Author: Robinson, DH; Liang, L-T; Oprandy, J; Nielsen, TB; Kang, Y-H
Abstract: The pathophysiologies of both decompression sickness (DCS) and gas embolism (GE) may involve ischemic injury to the microvessels of the central nervous system. We have isolated and cultured porcine cerebral microvascular (PCMV) endothelial cells in order to directly study the endothelial effects of ischemia. We have transformed PCMV endothelial cells using L cell-conditioned medium. We provide evidence that the L cell virus, a murine retrovirus, is the vector for this transformation. These transformed cells, designated RVTE, have properties of both macrophages and microvascular endothelial cells. Macrophage-like properties include pinocytosis of low density lipoprotein, Fc receptor-mediated phagocytosis, phagocytosis of bacteria and the expression of macrophage cytochemi.cal enzyme markers. Microvascular endothelial cell properties include the expression of FVIII:RAg and self-organization into capillary-like structures in vitro. This transformation may indicate that the cerebral microvascular endothelial cell is ontogenetically related to a hematopoietic and/or phagocytic precursor. The ischemic pathophysiology of both DCS and GE amy involve a similar macrophage-like transformation of the brain capillary endothelial cell. Accordingly, these new cell lines may serve as useful cell biology tools for in vitro investigations of DCS and GE. (Supported by NMRDC Work Unit MR04101.001-1055)
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Joint Annual Scientific Meeting with the International Congress for Hyperbaric Medicine and the European Undersea Biomedical Society held 11-18 August 1990. Okura Hotel, Amsterdam, The Netherlands (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/7280
Date: 1990

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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