[abstract] HOTBED OF PSEUDOMONAS SPECIES IN SATURATION DIVES IN THE DEEP DIVING SIMULATOR

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[abstract] HOTBED OF PSEUDOMONAS SPECIES IN SATURATION DIVES IN THE DEEP DIVING SIMULATOR

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Title: [abstract] HOTBED OF PSEUDOMONAS SPECIES IN SATURATION DIVES IN THE DEEP DIVING SIMULATOR
Author: Shinkai, M; Ohara, I; Suzuki, S; Ikeda, M; Hashimoto, A; Shinomiya, N; Ito, A
Abstract: BACKGROUND: Infectious disease caused by Pseudomonas species (P.S.) during saturation dive simulation has been a great distress to divers. There have been three possible contamination pathways during simulated saturation diving; contamination inside the chamber complex due to insufficient cleansing, contamination brought in after excursion dives and change of the indigenous microflora in diver's body. So far we have failed to completely eliminate infection with Pseudomonas species. In a 4.1 MPa simulated saturation diving operation conducted in 1997, we changed the cleaning method inside the chamber complex and tried to find out the main pathway of P.S. METHODS: To clean the inside of the chamber complex, we alternated three different disinfectants every four days during the dive; 0.05percent benzalkonium chloride, alkyldilyaminoethylglycine hydorchloride and chloheidine digluconate solution. We cultured bacteria in samples taken from all places where divers had chances to contact water, including the wet pot and hot water supply system to the umbilical hot water hoses. We also regularly cultured samples taken from the both external ears, nose and pharynx of six divers who participated in the dive. RESULTS: After the first excursion dive, P.S. was mostly found in the culture of a sampled water taken from the umbilical hot water hoses at which we have never looked. The number of P.S. was amounted to 61,000/ml of sampled water. After circulating hot water at 60 C for an hour for sterilization, the number was decreased to 0/ml. Thereafter the number reached to only 160/ml following the second excursion dive. Four out of six divers got infected with P.S. right after the first excursion dive, but none after the sterilization with hot water. CONCLUSION: It was found that the hot water supply system to umbilical hot water hoses was the hotbed of P.S. and that sterilizing thoroughly and frequently was important to decrease the number of P.S. in the saturation dive simulation.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/684
Date: 1998

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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