[abstract] DECOMPLEMENTATION REDUCES HEMODYNAMIC EFFECTS OF PULMONARY GAS EMBOLISM IN THE RAT.

Rubicon Research Repository/Manakin Repository

[abstract] DECOMPLEMENTATION REDUCES HEMODYNAMIC EFFECTS OF PULMONARY GAS EMBOLISM IN THE RAT.

Show full item record


Title: [abstract] DECOMPLEMENTATION REDUCES HEMODYNAMIC EFFECTS OF PULMONARY GAS EMBOLISM IN THE RAT.
Author: Chien, PS; Huang, KL; Kishi, Y; Lin, YC
Abstract: We investigated the involvement of complement system in the manifestation of hemodynamic effects of pulmonary gas embolism (PGE) in male Wistar rats. It is known that intravenous bubbles activate the complement system via an alternate pathway to produce anaphylatoxins C3a and C5a, which activate mast cells and basophils to release histamine, and leukocytes to produce reactive oxygen metabolites, thereby producing a depressed hemodynamic state and lung injury. PGE, which was induced by intravenous infusion of air at 9.6 ul/min for 30 min, elevated systolic right ventricular pressure (RVP) from 33 +- 2 mmHg to 45 +- 5 mmHg and raised systolic pulmonary vascular resistant (PVR) by 82%. PGE lowered mean arterial blood pressure (ABP) by 54%, cardiac output (CO) by 23%, heart rate (HR) by 19%, and the total peripheral resistance (TPR) by 40% at 10 min after air infusion. The lung water content 1 h post PGE was 79.56 +- 0.79 %, an increase of 3.0% from the control. All of the above changes were significant at p<0.01. After the pretreatment with 200 U/kg of cobra venom (CVF^h) 16 h prior, PGE lowered ABP by 23% (p<0.05), CO by 3% (p>0.05), HR by 1% (p>0.05), TPR by 19% (p<0.05); and increased RVP by 43% (pc0.01); PVR by 37% (p>0.05) and lung water content by 2.5% (p<0.01). Thus, after decomplementation, circulatory effects of PGE were reduced significantly (p<0.05) compared to the control group. Pretreatment with CVF^h by itself produced no significant changes (p>0.05) in circulatory functions. The respective values prior to PGE for the control (N = 6,382 +- 6 g) and treated rats (N = 6,381 +- 6g) were, RVP, 33 +- 2,36 +- 3 mmHg; ABP, 129 +- 12, 142 +- 15 mmHg; CO, 99 +- 13, 112 +- 22 ml/min; HR, 380 +- 30,384 +- 28 beat/min; TPR, 1.37 +- 0.13, 1.34 +- 0.28 mmHg/ml/min; PVR, 0.34 +- 0.04, 0.33 +- 0.05 mmHg/ml/min. The evidence suggests that decomplementation reduces hemodynamic effects of pulmonary gas embolism, but cannot prevent pulmonary hypertension and lung injury. (Supported in part by NOAA Sea Grant NA89AA-D-SG063 R/HP-6 and Leahi Trust).
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held June 23-27, 1992. Hyatt Regency Bethesda Hotel, Bethesda, Maryland (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/6475
Date: 1992

Files in this item

Files Size Format View
abstract.txt 258bytes Text file View/Open

This item appears in the following Collection(s)

  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

Show full item record

Browse

My Account