[abstract] WATER-SALT BALANCE OF THE MICE BRAINS UNDER CONDITIONS OF HELIOX HIGH PRESSURE.

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[abstract] WATER-SALT BALANCE OF THE MICE BRAINS UNDER CONDITIONS OF HELIOX HIGH PRESSURE.

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Title: [abstract] WATER-SALT BALANCE OF THE MICE BRAINS UNDER CONDITIONS OF HELIOX HIGH PRESSURE.
Author: Kostkin, VB; Leontjev, VG; Harenicheva, OL
Abstract: BACKGROUND. The investigation was undertaken to study mechanisms of HPNS development. METHODS. Two groups of 5 mice each were subjected to compression up to 36 and 71 ata, so that the total time under pressure was 50 min in both cases. Then the mice were frozen with liquid nitrogen in the barochamber. After explosive decompression in the whole brain content of water,ions Ha and K (atomic-absorbtive photometry) were estimated. In the synaptosomal fraction Na,K-ATPase were defined. Volume of the neurones of the nucleus caudatus were measured by means of ocular-micrometer in cxyostate sections staned after Nissl. RESULTS. At 36 ata (tremor) and at 71 ata (beginning of convulsion) content of water and ions did not change in comparison with control (air, 1 ata). The enzymatic activity at 36 ata dropped by 9%, and at 71 ata -by 21% ( in the control-21,99+-1,15 lDDlol p/mg of protein/h). The volume of neurones at 36 ata decreased by 19%, and at 71 ata -by 27% (in control 487+-35 mcm3 ). CONCLUSION. Lack of any changes in water and ions contents in the whole brain on the background of decreasing ATPase activity and neuron volumes can demonstrate certain disorders in redistribution of salt and liquid between neurones and extracellular medium when HPNS motor disorders develop.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held June 22-26, 1994. Westin Hotel, Denver, Colorado (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/5743
Date: 1994

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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