[abstract] PARENCHYMAL LUNG INJURY FOLLOWING SMOKE INHALATION: INHIBITION BY HYPERBARIC OXYGEN (HBO).

Rubicon Research Repository/Manakin Repository

[abstract] PARENCHYMAL LUNG INJURY FOLLOWING SMOKE INHALATION: INHIBITION BY HYPERBARIC OXYGEN (HBO).

Show full item record


Title: [abstract] PARENCHYMAL LUNG INJURY FOLLOWING SMOKE INHALATION: INHIBITION BY HYPERBARIC OXYGEN (HBO).
Author: Thom, SR; Mendiguren, I; Fisher, D
Abstract: BACKGROUND: The mechanism of smoke-induced parenchymal lung injury, versus solely airways inflammation, is unclear. Leukocyte (WBC) sequestration was necessary for alveolar injury in an rat model that replicates clinical injury patterns (Am Rev Resp Dis 149: 220, 1994). As HBO has been shown to inhibit WBC B2 integrin adhesion molecules, we investigated the effect of HBO in this smoke model. METHODS: Rats were exposed to smoke from smoldering douglas fir wood + polyvinyl chloride for 30 min. Leukocyte activation, required for smoke induced alveolar inflammation, was achieved with an i.P. injection of sterile oyster shell glycogen 4 h prior to smoke. HBO (3 ATA O2, 45 min) was given before, or immediately after glycogen + smoke, and lung studies were carried out 24 h later. Leukocyte sequestration was quantified as myeloperoxidase activity (MPO)in lung homogenates (A~/min/gm lung). Lung permeability was assessed as leakage of bovine serum albumin. RESULTS: MPO activity after glycogen+smoke was l3.9±l.4 (SE, n=l9), and permeability was 244±48% over control(*p<O .05, ANOVA). The control MPO value was 2.4±0.S (7). Smoke or glycogen, alone, caused an insignificant increase in MPO of 4.S±l.S(6), and 6.2±1.4 (4), respectively, and no increase in permeability. HBO treatment after glycogen+smoke resulted in an MPO activity of 7.8±l.0(7)[NS, not significant]. Treatment with HBO even before smoke+glycogen gave an MPO value of 6.7±1.2(7) [NS] and permeability of 90±4%(7) [NS] vs control. CONCLUSION: HBO significantly inhibited smoke-induced lung injury. Because pre-treatment was effective we conclude that the mechanism of action was related to modification of the host responses to the insult, and is consistent with an inhibition of leukocyte adhesion.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held June 22-26, 1994. Westin Hotel, Denver, Colorado (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/5689
Date: 1994

Files in this item

Files Size Format View
abstract.txt 259bytes Text file View/Open

This item appears in the following Collection(s)

  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

Show full item record

Browse

My Account