[abstract] ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM THE AQUATIC ENVIRONMENT: IMPLICATIONS FOR CLINICAL THERAPY.

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[abstract] ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM THE AQUATIC ENVIRONMENT: IMPLICATIONS FOR CLINICAL THERAPY.

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dc.contributor.author Auerbach, P
dc.contributor.author Yajko, D
dc.contributor.author Hadley, K
dc.contributor.author Kizer, K
dc.date.accessioned 2008-01-08T02:28:24Z
dc.date.available 2008-01-08T02:28:24Z
dc.date.issued 1985
dc.identifier.citation Undersea Biomedical Research, Vol. 12, No. 1 Supplement, March 1985 en
dc.identifier.uri http://archive.rubicon-foundation.org/5505
dc.description Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held 11-14 June 1985. The Hyatt Regency Hotel, Long Beach, California (http://www.uhms.org) en
dc.description.abstract Ocean water and tissue samples were obtained from a variety of sources with phylogenetic (shark, fire coral, sea urchin) and geographic (California, Egypt) disparity. These were cultured in Heart infusion, Brucella, Cohunbia, TCBS, Middlebrook 7H1O and 7Hll, and Sabouraud agars, as well as Lowenstein Jensen nedium. Agars were prepared in either distilled water or from ocean water. Plates were incubated in air at room temperature. After isolation of purified colonies, identification procedures were performed. 1SI slants and oxidation/ fermentation tubes were inoculated, and the following tests were performed: growth on TCBS and MacConkey agar; growth on trypticase soy blood agar without sea water; nitrate reduction; 0/129 sensitivity; luminescence; Thornley's arginine; lysine and ornithine decarboxylase; growth at 42 degrees C.; growth in 0,3,6,8 and 10'/0 NaCl; V-P reaction; gas from glucose; acid production from L-arabinose, m-inositol, D-mannose, and sucrose; production of amylase, gelatinase, and lipase. Thirty-three isolates of Vibrio species (including V. harveyi, V. pelagius I and II, V. anguillarum, V. algioolyticus, V. damsela and V. fluvialis II), 2 isolates of Pbotobacteriurn angpstum, and 23 isolates of non-fermentative marine bacteria were identified and characterized. Three gram positive isolates were also isolated. Antibiotic susceptibility testing using 21 different ant~crobials was carried out in rrdcrotiter plates containing antibiotics in Mueller-Hinton broth containing 2.~k NaCl. Analysis of the antibiograms showed that "while the non-fermentative marine bacteria were generally susceptible to the antibiotics tested, marine Vibrio species tended to be relatively resistant to a wide variety of antirrdcrobials, including 1st, 2nd, and 3rd generation cephalosporins. Antibiotics effective against all species included chlorarrphenicol, trirrethoprirn/sulfarrethoxazole, and thienamycin. Other antibiotics which were effective (i.e., only one isolate was resistant) included tetracyline, azthreonam, cefoperazone, and ceftazidirre. Some isolates failed to grow in the medium used for susceptibility testing. Commercial test kits do not yield accurate identifications of bacteria, the acquisition of antimicrobial susceptibility data on these organisms gains added importance. en
dc.format.extent 259 bytes
dc.format.mimetype text/plain
dc.language.iso en en
dc.publisher Undersea and Hyperbaric Medical Society, Inc. en
dc.subject Hyperbaric Oxygenation en
dc.subject antibiotics en
dc.subject V. harveyi en
dc.subject V. pelagius en
dc.subject V. anguillarum en
dc.subject V. algioolyticus en
dc.subject V. damsela en
dc.subject V. fluvialis en
dc.subject bacteria en
dc.subject aquatic environment en
dc.title [abstract] ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM THE AQUATIC ENVIRONMENT: IMPLICATIONS FOR CLINICAL THERAPY. en
dc.type Other en

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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