[abstract] SPIN TRAPPING AND SPIN LABELING STUDIES OF OXYGEN TOXICITY.

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[abstract] SPIN TRAPPING AND SPIN LABELING STUDIES OF OXYGEN TOXICITY.

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Title: [abstract] SPIN TRAPPING AND SPIN LABELING STUDIES OF OXYGEN TOXICITY.
Author: Tan, CT; Tan, KS; Hsia, JC
Abstract: Recently, underwater exploration and construction by man have led to discoveries of physiological phenomena such as nitrogen narcosis and oxygen toxicity. Oxygen toxicity is believed to be a manifestation of membrane damage by several known reactive intermediates such as OH., O2. etc. We have studied the reactivity and identity of these toxic reactive intermediates in lipid bilayer model membranes by means of spin trapping and spin labeling techniques. In the presence of molecular oxygen, the unsaturated fatty acids of cardiolipin tend to initiate an auto-oxidation reaction. These autocatalytic free radical reactions generate many highly reactive and potentially damaging chemical species. These reactive intermediates can oxidize 4-hydroxy-2,2,6,6-tetramethylpiperidine, a spin label precursor to 4-hydroxy-2,2,6,6-tetramethylpiperidinoxy which can be monitored and characterized by electron spin resonance (esr) spectroscopy. Factors (e.g. Ca++, cytochrome C, time etc.) affecting the reactive intermediates have been studied by esr technique. The spin trap, 5,5-dimethyl-1-pyrroline-1-oxide is applied to investigate the primarily free radicals produced from the auto-oxidation of cardiolipin. At least three different radicals are generated in the model membranes. Polymerization is observed when cytochrome C is complexed with cardiolipin (20 percent by wt) in phosphatidyl-choline model membranes.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held April 28-May 1, 1978. Washington Plaza Hotel, Seattle, Washington (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/4224
Date: 1978

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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