[abstract] OXYGEN FREE RADICAL PRODUCTION DURING CARBON MONOXIDE POISONING: INFLUENCE OF OXYGEN PRESSURE DURING REOXYGENATION: PRELIMINARY RESULTS

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[abstract] OXYGEN FREE RADICAL PRODUCTION DURING CARBON MONOXIDE POISONING: INFLUENCE OF OXYGEN PRESSURE DURING REOXYGENATION: PRELIMINARY RESULTS

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Title: [abstract] OXYGEN FREE RADICAL PRODUCTION DURING CARBON MONOXIDE POISONING: INFLUENCE OF OXYGEN PRESSURE DURING REOXYGENATION: PRELIMINARY RESULTS
Author: Mathieu, D; Cotelle, N; Neviere, R; Marechal, X; Lhermitte, M; Lefebvre-Lebleu, N; Wattel, F
Abstract: The occurrence of an overproduction of oxygen free radicals (OFR) during ischemia-reoxygenation injury is actually well known and the risk of hyperoxia during reoxygenation has been stressed. Recently, OFR overproduction during carbon monoxide (CO) poisoning has been reported contrasting with the fact that hyperoxygenation lowers this OFR production. A study was undertaken to explain this paradox. METHOD: Sprague-Dawley rats are intoxicated by exposition to a CO enriched atmosphere (3.000 ppm) during 30 minutes, then reoxygenated during 50 minutes either in atmospheric air, normobaric pure oxygen or 2.5 ATA hyperbaric pure oxygen in a randomized fashion. A control group and a group sacrificed after CO poisoning are also considered. Immediately after, the brain is dissected and homogenized under a pure N2 atmosphere. OFR detection is made by ESR (Varian E 109), dosage of ascorbic and dehydroascorbic acid, reduced and oxidized gluthatione, xanthine and hypoxanthine, malonyldialdehyde by HPLC, SOD activity by spectrophotometry. Data are analyzed by ANOVA. RESULTS: In the group of animals killed after CO poisoning, a significant decrease exists in the ESR signal corresponding to the ascorbyl radical (69 +/- 11 vs. 100 +/- 17 nM; p , 0.05). A decrease is also seen in biochemical determinations and is concomitant with a decrease in reduced to oxidized glutathione ratio and with an increase in SOD activity. For the 3 groups studied after reoxygenation, these findings are less important in the 2 groups with the highest oxygen pressure even if the small number of animals precluded definitive interpretation. CONCLUSION: OFR production seems to occur mainly during the CO poisoning phase and to be cleared quicker during oxygen enriched reoxygenation phase than in air.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/316
Date: 1997

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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