[abstract]A2A ADENOSINE RECEPTOR AND CFOS/CJUN EXPRESSION IN BRAIN OF FREE-MOVING RATS SUBMITTED TO HYPERBARIC HYPEROXIA

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[abstract]A2A ADENOSINE RECEPTOR AND CFOS/CJUN EXPRESSION IN BRAIN OF FREE-MOVING RATS SUBMITTED TO HYPERBARIC HYPEROXIA

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Title: [abstract]A2A ADENOSINE RECEPTOR AND CFOS/CJUN EXPRESSION IN BRAIN OF FREE-MOVING RATS SUBMITTED TO HYPERBARIC HYPEROXIA
Author: Bruzzese L; Gariboldi V; Nee L; Condo J; Fenouillet E; Ruf J; Lucciano M; Guieu R; Rostain JC
Abstract: INTRODUCTION/BACKGROUND: c-Fos, a cellular proto-oncogene upregulated by many extracellular signals including stress, dimerises with c-Jun to form the AP-1 transcription factor, which upregulates transcription of a diverse range of genes involved in consequences to defence against invasion and cell damage. Upregulation of c-Fos or c-Jun mRNA in a neuron indicates recent activity. Adenosine receptors (A2AR) are implicated in cardiovascular system regulation, expressed in a brain stem area implicated in blood pressure control and in brain ganglia implicated in motor control regulation via the dopamine D2 receptors. The aim of this study was to evaluate the influence of hyperbaric hyperoxia on c-Fos/c-Jun and A2AR expressions in rat brains. MATERIALS AND METHODS: Male Sprague Dawley rats were exposed in a pressure chamber for four hours at 2 atm abs of oxygen (temperature 25/26 degreesC; H20: 40-60 percent; CO2: less than 300 ppm). After exposure, brains (n=6+6 controls) were taken and frozen at -70 degrees C, quickly thawed at 37 degrees C and solubilized with 4 percent SDS aqueous solution by 30-minute sonication at 47 kHz. After protein quantification, 100 mg of murine brain solubilisates were submitted to standard electrophoresis procedure. Separated proteins in 12 percent acrylamide minigel were electrotransferred onto a PVDF membrane. Blotted membrane was placed into the blot holder of the SNAP i.d. protein detection system, saturated with non-fat dried milk and incubated 20 minutes with one of the appropriately diluted mouse monoclonal antibodies, anti-A2AR, anti-c-Fos and anti-c-Jun. Blots were visualized by horseradish peroxidase labeled anti-mouse IgG Fab specific antibodies and enhanced chemilumine-scence substrate using a Kodak Image Station 440CF. The staining intensities of the bands were measured densitometrically using public-domain NIH Image software. RESULTS: c-Fos (+320±70 percent) and c-Jun (+450±89 percent) expression were increased and A2AR expression was decreased (-76±14 percent) in brain of rats exposed to hyperbaric hyperoxia compared to control. SUMMARY/CONCLUSIONS: The decrease in brain A2AR expression may participate to the change of dopa-minergic activity recorded in hyperoxia. The precise consequence of the increase in c-Fos/c-Jun expression during hyperoxia needs further investigations.
Description: Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/10650
Date: 2013

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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