[abstract]ADENOSINE COULD PROTECT AGAINST HYPERBARIC OXYGEN EXPOSURE-INDUCED CONVULSION

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[abstract]ADENOSINE COULD PROTECT AGAINST HYPERBARIC OXYGEN EXPOSURE-INDUCED CONVULSION

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Title: [abstract]ADENOSINE COULD PROTECT AGAINST HYPERBARIC OXYGEN EXPOSURE-INDUCED CONVULSION
Author: Li, RP; Sun, YW; Chen, YL; Xu, WG; Liu, SL; Cai, ZY
Abstract: Introduction/Background: Prolonged exposure to hyperbaric oxygen (HBO2) can lead to seizure, the etiology of which is not completely understood. Adenosine (Ado) plays an inhibitory role in the central nervous system (CNS). In the present study we investigated the effects of HBO2 on the content of GAD in mice brains, and the effects of exogenous Ado on HBO2-induced seizure. Materials and methods: BALB/C mice were exposed to pure oxygen at 500 kPa for 10 minutes, then they were decapitated; the cerebrum was separated and homogenized. The content of Ado, ATP, ADP and AMP were determined using the HPLC method. In another experiment, mice were administered with Ado by intracerebroventricular injection (ICV) 10 minutes before a 500 kPa HBO2 exposure. Seizures were determined by behavior observation, and time to the first occurrence of generalized seizure was recorded as latency after the chamber pressure reached 500 kPa. In both experiments, control mice were exposed to 500 kPa hyperbaric environments, but the O2 partial pressure was kept normal, as that in the atmosphere. Results: Compared with control, Ado in the cerebrum treated with HBO2 was increased markedly. Meanwhile, the ATP content was decreased significantly, and the contents of ADP and AMP were also reduced. Ado administered directly into the brain could prolong the latency dramatically, reached to 17.81±1.55, 23.26±1.74, 29.34±2.07 minutes when injected at the doses of 2.5, 5, 10 ?g/site respectively, while the latency of control was 9.76±1.28 minutes. Summary/Conclusions: Ado in the CNS could rapidly respond to HBO2 exposure, and it has a noticeable effect of prolonging HBO2-induced seizures. As an innate substrate and a kind of neuromodulator with inhibitory effect in the brain, its potentiality in preventing CNS oxygen toxicity is worth further investigations.
Description: Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/10465
Date: 2012

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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